Fluoreszenzproteine messen intrazelluläre Sauerstoffkonzentrationen

Abstract Hypoxia is a hallmark of many pathologies including solid tumors. In basic research, analysis hypoxia by light microscopy became feasible through development genetically encoded reporters. However, the dependence fluorescent proteins on molecular oxygen for maturation limits their use in hypoxic tissue. Overcoming this limitation, family hypoxia-sensors based oxygen-independent protein UnaG was developed research group Friedemann Kiefer.